human β ngf  (R&D Systems)

Journal: Journal of Genetic Engineering & Biotechnology

Article Title: Influence of growth factors and different supplements on metabolic response and productivity of IgA-producing CHO cell lines

doi: 10.1016/j.jgeb.2025.100588

Figure Lengend Snippet: Influence of FGF-b, hNGF and hEGF on metabolites level and IgA1- IgA2m1 stable cell lines productivity on 10 days of static culture.

Article Snippet: The medium was supplemented with growth factors such as recombinant human epidermal growth factor (EGF), recombinant human nerve growth factor (β-NGF), and recombinant human fibroblast growth factor (FGF-b) (all Sino Biological, China) as growth stimulants. hFGF-b solution was introduced to final concentrations of 11 ng/ml and hEGF, hNGF solutions were added to a final concentration of 12.5 and 25 ng/well.

Techniques: Stable Transfection

Journal: Nature Communications

Article Title: Discovery of an ITK and TRK kinase inhibitor for the potential topical treatment of atopic dermatitis

doi: 10.1038/s41467-026-70000-6

Figure Lengend Snippet: Treatment of skin explants with PF-07245303 can reverse the disease signature. a , b Scatterplot of RNA expression differences in anti-CD3/CD28 and Th2 cytokine-stimulated and unstimulated skin explants. Each dot represents a distinct gene. a Red dots represent genes that are induced by stimulation with p < 0.05, and blue dots represent genes that are reduced by stimulation with p < 0.05. b Dots are colored based on regulation in atopic dermatitis skin tissue versus healthy control from GEO dataset GSE121212 . c Scatterplot of RNA expression differences in skin explants treated with PF-07245303 and then stimulated with anti-CD3/CD28 and Th2 cytokines. Dots are colored based on the regulation in ( a ). d Expression of RNA in untreated ( n = 12), anti-CD3/CD28/Th2 stimulated ( n = 12), and anti-CD3/CD28/Th2/PF-07245303 ( n = 12) treated skin explants for CXCL11 , IFNG , IL13 , IL4 , IL17A , IL2 , FLG , FLG2 , IL31RA , NGF , OSMR, and IL4R from RNA sequencing studies. Values on the y-axis represent EdgeR normalized expression. Comparisons used Dunn’s tests and p -values are adjusted for multiple comparisons using the Holm–Bonferroni method. Data are presented as box plots (center line at the median, upper bound at 75th percentile, lower bound at 25th percentile) with whiskers at minimum and maximum values. Each dot represents one donor with 3 technical replicates per donor. Source data are provided as a file.

Article Snippet: The mixture was placed in an incubator at 37 °C with 5% CO 2 for 30 min, followed by stimulation with recombinant human NGF (R&D Systems, 256-GF) at a final concentration 0.1 μg/mL (or PBS as negative control) and the resulting mixtures were further incubated for 20 min. Next, anti-CD123-APC (Miltenyi Biotec, 130-115-265) and anti-CD203c-PE (Miltenyi Biotec, 130-092-343) antibodies were added and continued to incubate for another 15 minutes.

Techniques: RNA Expression, Control, Expressing, RNA Sequencing